Radioimmunoassay of Human Fibrinopeptide A

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Fibrinogen in the Radioimmunoassay of Human Fibrinopeptide

A radioimmunoassay (RIA) technique has been devised for the measurement of human fibrinopeptide A (PA). The system utilizes rabbit antiserum to native human PA and a synthetic fibrinopeptide, with tyrosine substituted for phenylanine in amino acid position 8. The test detects native human PA at a concentration of 0.1 ng/ml, but does not cross react with human flbrinopeptide B or with flbnnopept...

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Fibrinogen in the Radioimmunoassay of Human Fibrinopeptide A

A radioimmunoassay (RIA) technique has been devised for the measurement of human fibrinopeptide A (PA). The system utilizes rabbit antiserum to native human PA and a synthetic fibrinopeptide, with tyrosine substituted for phenylanine in amino acid position 8. The test detects native human PA at a concentration of 0.1 ng/ml, but does not cross react with human flbrinopeptide B or with flbnnopept...

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Radioimmunoassay of human fibrinopeptide B and kinetics of fibrinopeptide cleavage by different enzymes.

Thrombin converts fibrinogen to fibrin monomer by cleaving fibrinopeptides A and B (FPA and FPB) from the amino terminal ends of the A (alpha) and B (beta) chains. A radioimmunoassay capable of measuring the A peptide in human blood as an index of thrombin action in vivo has been described previously. This paper describes the development of a radioimmunoassay for FPB and the use of both assays ...

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Reaction of plasmic degradation products of fibrinogen in the radioimmunoassay of human fibrinopeptide A.

A radioimmunoassay (RIA) technique has been devised for the measurement of human fibrinopeptide A (FPA). The system utilizes rabbit antiserum to native human FPA and a synthetic fibrinopeptide, with tyrosine substituted for phenylanine in amino acid position 8. The test detects native human FPA at a concentration of 0.1 ng/ml, but does not cross react with human fibrinopeptide B or with fibrino...

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Measurement of fibrinopeptide A in human blood.

Since thrombin cleaves fibrinopeptides A (FPA) and B from the NH(2)-terminal end of the fibrinogen molecule, measurement of fibrinopeptide levels in plasma may provide a direct index of thrombin action. Recently a radioimmunoassay for FPA has been developed, and in the present paper, we describe the application of this assay to the measurement of FPA levels in clinical blood samples. Since fibr...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1971

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.68.10.2350